Résumé
I have six years of post-doctoral research experience in the field of malaria, precisely investigation on mosquito vectors and Plasmodium parasites interactions. My post-doctoral research includes a first position at the Institut de Recherche pour le Développement (IRD) of Montpellier from Nov 2013 to Dec 2016 and a second position at the Institut Pasteur-France since January 2017. During my M. Sc (2008-2010; UCAC-Yaounde-Cameroon) and PhD (2010-2013; IRD-Montpellier) I investigated the nature of interactions between Plasmodium falciparum and Anopheles gambiae as main vector of malaria. Previously, I got a B. Sc (2002) and a M. Sc in Biochemistry (2003) Next followed a training as Technician in biomedical analysis (2006-2009). My academic career so far led to (3) scientific papers as main author and (11) as co-author with multiple field works in Madagascar and Cameroon for sample preparation. My ultimate goal is to have a permanent research position which will be a springboard for my future career as a woman in this competitive field. While waiting for this opportunity to be offered to me, I am following a training course in project management
Expériences professionnelles
Post-doctorat
Institut Pasteur , Paris - CDD
De Janvier 2017 à Juillet 2020
Project: Proteo-transcriptomics of Anopheles - Plasmodium vivax interactions towards identification of malaria transmission blocking targets (Funding: Labex IBEID). The main accomplished goals were:
The design of the experimental protocols and the production of biological samples which implied two missions in the field (2017-2018-Madagascar): parasitological survey for the selection of parasites, experimental infection and preparation of mosquito midguts for RNAseq and Proteomic analyses;
RNAseq analysis with the collaboration of Etienne Kornobis (C3BI-Institut Pasteur)
The Proteomic analyis with the collaboration of Proteomic platform (Institut Pasteur) and Etienne Kornobis-C3BI is underway. We already assessed the experimental conditions suitable for sample preparation in the field in Madagascar. All samples have been prepared in 2018 and 2019.
Supervision of Amgen Scholar: St Catharine's College Cambridge, CB1 2RL. Institut Pasteur (the student won the poster competition)
Contribution to the Practical Work of the Insect Vectors and Transmission of Pathogens Course: Session of April 2018; Institut Pasteur.
Supervision of Master 1, École Pratique des Hautes Études-Paris.
Supervision of L3, Université de Poitiers.
Post-doctorat
IRD , Montpellier - CDD
De Novembre 2013 à Décembre 2016
• Investigation of virulence factors from two E. coli strains (444ST95 and 351ST73) isolated in mosquito midgut: 444ST95 drastically reduces the development of P. falciparum whereas 351ST73 shows no effect when comparing to control group. Experiments were carried out at CPTP
- The E. coli clones isolated from mosquito midguts were compared by associating two molecular typing methods: an “Enterobacterial Repetitive Intergenic Consensus” sequence (ERIC) and a “Random Amplified Polymorphic DNA” (RAPD)-PCR, then submitted for typing by multilocus sequence typing (MLST)
- The detection of the E. coli associated virulence genes was then assessed by PCR
• Setting up the genotyping technique to characterize Plasmodium falciparum malaria parasites haplotypes during infection in mosquito tissues using the Illumina Miseq new generation sequencing (IRD-MIVEGEC).
Co-supervision of Master and PhD students from Cameroon: IRD-MIVEGEC-Montpellier and LRP-OCEAC-Cameroon between 2011 and 2013
Formations complémentaires
Doctorat
University of Montpellier - Parasitology/Microbiology
2010 à 2013
Organization and managment of field work in Cameroon;
Collection of larval and pupal stages of the mosquito malaria vector Anopheles gambiae;
Identification of bacteria flora from mosquito larval and pupal stages using the chromogenic medium developped by Biomerieux-France; Genomic DNA extraction and sequencing of the 16S rDNA using the Sanger method;
Production of adult mosquito (F0) from the field collection and experimental infections using P. falciparum natural isolate;
Dissection of (F0) mosquito tissues (midguts, ovaries and salivary glands) 24 hours, 7 and 14 days post infection to assess the bacterial flora composition
Genomic DNA extraction from different mosquito tissues; sequencing of the V3-V4 hypervariable region of the 16S rRNA using 454 pyrosequencing;
Mosquito immune gene expression following Plasmodium infection: RNA extraction, Reverse transcription and RTqPCR analyses.
Master
Catholic University of Central Africa in Yaounde (UCAC), Cameroon - Microbiology-Immunology
2008 à 2010
Identification of natural bacteria from mosquito midgut using TGGE (Temperature gradient gel electrophoresis) technique and the Sanger sequencing method.
Investigate the nature of interaction between bacterial flora and Plasmodium infection in the mosquito midgut using experimental infections.
Maitrise
University of Yaounde - Biochemistry
2002 à 2003
Technicien en Analyses biomedicales
cath - Analyses biomédicales
2006 à 2009
Mastère spécialisé
CESI SAINT ETIENNE DU ROUVRAY - Management par projets
2020 à 2021
Management d'équipe
Gestion du projet (élaboration et suivi d'un plan de management)
Culture de projet, conduite de changement,
Amélioration des projets.
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